December 2011

Source: ISB News Report - December 2011
by Hiroaki Saika and Seiichi Toki

Gene targeting (GT) via homologous recombination is a powerful transformation technology not only for the analysis of the function of the gene of interest but also for the molecular breeding of crops, because it enables modification of the endogenous target gene as expected. Site-directed mutagenesis via GT without using exogenous positive selection markers is a 'clean' transformation technology. Moreover, the technique is generally more efficient than conventional mutation breeding, which depends on natural variation or induced random mutagenesis. Here, we describe the successful production of rice plants via GT that accumulate high levels of free tryptophan in mature seeds. We also discuss the potential of designed-mutation breeding in crops via GT.

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Website: http://www.virginia.edu

Published: January 1, 2012