Applications of impedance flow cytometry in doubled haploid technology
Switzerland
November 21, 2021
Doubled Haploid Technology Volume 3
by Springer Protocols
Doubled haploid (DH) technology is a very convenient approach in plant breeding to save a considerable amount of time, and the corresponding costs, to produce pure, fully homozygous parental lines, essential for large-scale production of hybrid seed. This is the main reason why it is widely used in private companies and academia to produce homozygous individuals.
The new “Doubled Haploid Technology” Volume 3 by Springer Protocols compiles the knowledge of experts in the production of DHs in different species.
The book features a chapter on “Applications of Impedance Flow Cytometry in Doubled Haploid Technology”. In this chapter concepts for identifying critical success factors in a DH protocol are presented. It also shows how an early microspore response can be monitored by simple impedance flow cytometry and used to optimize a DH protocol based on androgenesis.
Get the book on Springer.
More solutions from: Amphasys AG
Website: https://amphasys.com/ Published: December 21, 2021 |